Abstract

The techniques considered are paper and thin layer chromato graphy, column chromatography using a liquid mobile phase, and automatic analysis. Applications of paper chromatography to vegetable tannins, dyestuffs, and protein hydrolysates are considered, as are those of thin layer chromatography to lipids, amino acids and their derivatives, dyestuffs, and polyphenols. Gradient elution is men tioned in connection with column chromatography. where cellulosic ion exchangers have become prominent for the separation of high molecular weight substances, such as proteins. The latest development lies in taking advantage of the molecular sieve effect using cross-linked dextran gels (Sephadex), but there are problems when this technique is applied to the study of collagen. The principal method of amino acid analysis is still based on ion exchange resin columns, but it has now been automated. The apparatus available for this purpose can readily be used for other colorimetric determinations.